Sequence ID: DB070O21_F1.ab1

Motif: (GCAT)3 Motif length: 4 SSR: GCATGCATGCAT SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 512 21 54.69 42.86 6.00 2.00 TCGATGATCAGAGGAGTACAT 170 891 891 4.00 1.00   REVERSE 681 21 55.07 42.86 2.00 2.00 GGTCTTTCTCTTTCTCTTTCG Primer view: 1 TCCCTCCCAAGGAGTTGACACAAAGTGAAAGGTGTTCTATATATCTATCTATCCATCCAC 61 CAGGAATATTATGTATGCATCCACAGCCTCTACATACACTGCGCTTTCTTTTGAAACGAA 121 CAGAGCCTAAACTGTCTGTCCCCTTATTTTGACCAGGCCATCCACATCTTTGACTTGTTG 181 TCTAGTCCACTTTGAACTTATAGTCTCCCGAACATGTTACTGCAGGGAACTAATTATTAA 241 TCCACTTAAACCGGCAGGAATTGTTACCGTGCTTCAATACATAATCGGATGCTTCTACTG 301 ACTGATTTTTTTCAGTGGCAATTGATATTATGCTATACTTGTATTGTTGCCTTGTTGGCA 361 GTCATGTATGAACGTAGTAGGGCTGAACATGTATGCATTCAAGTTTTGAACCGGAACCTT 421 GGCGCCCGATTCGAGAAGAGGGCTGGGAAAGCAAAAGAAAAGCGCATGCATATATGTGCG 481 GCTTTGTGCTTAAAAGGCAGAGATCGATCGATCGATGATCAGAGGAGTACATGTTCGTCT                                   >>>>>>>>>>>>>>>>>>>>>        541 ATATATAAAACAAAAAGAGAAAAATAAAGGAAGAGATCGAGCAGAGGGAGGAGCAGGAAC 601 TTTGGGCATGCATGCATCGGTCTGGTCTGGTGCTGCACTTTGGTCTTTGGTCTTTGGTGC                                                              < 661 CGAAAGAGAAAGAGAAAGACCTCTCCTGGTATCGATCCCTGATGGATGGATGGATGCGAT   <<<<<<<<<<<<<<<<<<<<                                         721 TCTCTTCTCCTTTCCTTTTTTCTCTTTTGGCAGAGATATAGTGTAGCACCTCTCTCCTGG 781 TATCCATCCGATTAAAGCCGGCTAAAGAGGTTGATCATGATGAGAATAGAAACAAAACTA 841 AAATGTGTACTGATGTCACACGCTTCTTCCGTTGATGCTCTGGATCGATCG Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0