Sequence ID: DB070O21_F1.ab1

Motif: (GATG)4 Motif length: 4 SSR: GATGGATGGATGGATG SSR length: 16 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 668 21 54.89 47.62 4.00 2.00 GAAAGAGAAAGACCTCTCCTG 152 891 891 6.00 1.00   REVERSE 819 20 54.87 45.00 6.00 1.00 CATGATCAACCTCTTTAGCC Primer view: 1 TCCCTCCCAAGGAGTTGACACAAAGTGAAAGGTGTTCTATATATCTATCTATCCATCCAC 61 CAGGAATATTATGTATGCATCCACAGCCTCTACATACACTGCGCTTTCTTTTGAAACGAA 121 CAGAGCCTAAACTGTCTGTCCCCTTATTTTGACCAGGCCATCCACATCTTTGACTTGTTG 181 TCTAGTCCACTTTGAACTTATAGTCTCCCGAACATGTTACTGCAGGGAACTAATTATTAA 241 TCCACTTAAACCGGCAGGAATTGTTACCGTGCTTCAATACATAATCGGATGCTTCTACTG 301 ACTGATTTTTTTCAGTGGCAATTGATATTATGCTATACTTGTATTGTTGCCTTGTTGGCA 361 GTCATGTATGAACGTAGTAGGGCTGAACATGTATGCATTCAAGTTTTGAACCGGAACCTT 421 GGCGCCCGATTCGAGAAGAGGGCTGGGAAAGCAAAAGAAAAGCGCATGCATATATGTGCG 481 GCTTTGTGCTTAAAAGGCAGAGATCGATCGATCGATGATCAGAGGAGTACATGTTCGTCT 541 ATATATAAAACAAAAAGAGAAAAATAAAGGAAGAGATCGAGCAGAGGGAGGAGCAGGAAC 601 TTTGGGCATGCATGCATCGGTCTGGTCTGGTGCTGCACTTTGGTCTTTGGTCTTTGGTGC 661 CGAAAGAGAAAGAGAAAGACCTCTCCTGGTATCGATCCCTGATGGATGGATGGATGCGAT           >>>>>>>>>>>>>>>>>>>>>                                721 TCTCTTCTCCTTTCCTTTTTTCTCTTTTGGCAGAGATATAGTGTAGCACCTCTCTCCTGG 781 TATCCATCCGATTAAAGCCGGCTAAAGAGGTTGATCATGATGAGAATAGAAACAAAACTA                      <<<<<<<<<<<<<<<<<<<<                      841 AAATGTGTACTGATGTCACACGCTTCTTCCGTTGATGCTCTGGATCGATCG Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0