Sequence ID: DB070O21_F1.ab1

Motif: (GAAAGA)3 Motif length: 6 SSR: GAAAGAGAAAGAGAAAGA SSR length: 18 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 636 20 54.41 40.00 2.00 0.00 CACTTTGGTCTTTGGTCTTT 148 891 891 4.00 0.00   REVERSE 783 21 55.08 52.38 3.00 2.00 ATACCAGGAGAGAGGTGCTAC Primer view: 1 TCCCTCCCAAGGAGTTGACACAAAGTGAAAGGTGTTCTATATATCTATCTATCCATCCAC 61 CAGGAATATTATGTATGCATCCACAGCCTCTACATACACTGCGCTTTCTTTTGAAACGAA 121 CAGAGCCTAAACTGTCTGTCCCCTTATTTTGACCAGGCCATCCACATCTTTGACTTGTTG 181 TCTAGTCCACTTTGAACTTATAGTCTCCCGAACATGTTACTGCAGGGAACTAATTATTAA 241 TCCACTTAAACCGGCAGGAATTGTTACCGTGCTTCAATACATAATCGGATGCTTCTACTG 301 ACTGATTTTTTTCAGTGGCAATTGATATTATGCTATACTTGTATTGTTGCCTTGTTGGCA 361 GTCATGTATGAACGTAGTAGGGCTGAACATGTATGCATTCAAGTTTTGAACCGGAACCTT 421 GGCGCCCGATTCGAGAAGAGGGCTGGGAAAGCAAAAGAAAAGCGCATGCATATATGTGCG 481 GCTTTGTGCTTAAAAGGCAGAGATCGATCGATCGATGATCAGAGGAGTACATGTTCGTCT 541 ATATATAAAACAAAAAGAGAAAAATAAAGGAAGAGATCGAGCAGAGGGAGGAGCAGGAAC 601 TTTGGGCATGCATGCATCGGTCTGGTCTGGTGCTGCACTTTGGTCTTTGGTCTTTGGTGC                                       >>>>>>>>>>>>>>>>>>>>     661 CGAAAGAGAAAGAGAAAGACCTCTCCTGGTATCGATCCCTGATGGATGGATGGATGCGAT 721 TCTCTTCTCCTTTCCTTTTTTCTCTTTTGGCAGAGATATAGTGTAGCACCTCTCTCCTGG                                             <<<<<<<<<<<<<<<<<< 781 TATCCATCCGATTAAAGCCGGCTAAAGAGGTTGATCATGATGAGAATAGAAACAAAACTA   <<<                                                          841 AAATGTGTACTGATGTCACACGCTTCTTCCGTTGATGCTCTGGATCGATCG Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0