Sequence ID: DB096B10_R1.ab1

Motif: (GCC)4 Motif length: 3 SSR: GCCGCCGCCGCC SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 50 19 54.65 57.89 1.00 1.00 CACAGAGGGAGGAAGAGAC 129 928 928 4.00 1.00   REVERSE 178 19 54.55 52.63 6.00 2.00 GGATCCTGGAGGCTATATG Primer view: 1 TCGACTCTGAGGATCCCCTCAGCCCAGGCCATCAGGCCCAATACGCAGGCACAGAGGGAG                                                     >>>>>>>>>> 61 GAAGAGACCGGTCGAACGGGACTCCCGACTACTGCTTGCTGTGTGCGTGTGGGACCCGAG   >>>>>>>>>                                                    121 AGGCCGCCGCCGCCGCAGTCCGCCGCCGCCGCCGCCGCTCATATAGCCTCCAGGATCCCC                                          <<<<<<<<<<<<<<<<<<<   181 CCGTACTCCCTCGCATCGCCTAGTCAGATGCGGCCGGCGGCTTCCCCCGTCCCCGCCCGC 241 GGCGCTAGGTCGCCGCCAGCTTGGCCCTGAGCTCGCCGTCCTGCAGTCCTGCCTCCCTGC 301 CCTGCCCCGGTGCTCCCCCGACTCGGCGACTCCCACTTCCAGTACTCAATCGGCTTCGCC 361 CCAATCGGTAATGCGCACTTTCCTCCCACTTTGCTTGCTCATCTGCGGCTCTGCCCACTG 421 CTCTCTCCCCCCAAAATCTCTGTTCTTTAGTTCAGGACTACGGAGTACTTGATGACTGTA 481 CAAATCAAAGTATTGTGTGATGAGTCGAGTACAAATCAAATGAATATGTGATGAATCGAT 541 ATTCCCCTTCGAGAATTTGTTGTTCCGGTTTAATTTTGCACAAATCAAGTGAATATGTGA 601 TGAGTCGAACGTCTTGAAGATTTAATCACGGGGGCCTCGGATCAATCAACCCTTTGATTT 661 GGTATGTTGTCTTTTGCTATCTACAGTATTACATCAATAGTTGAACCATTTAATATTTGC 721 GTCCAAATAATTAGCAATTATCTTGGAATTATTTGGCCCCGGGTGGGAGCATTCCCTGGC 781 TCTGCTGTTGGGTTCAGTCGCCCTGTGGAGTGTGGCCTACTCCTATGTCAGGAGAGGGAG 841 TGAGCGAGGGGTTGATCAGTCGTTCGAGCCCGATGATGAATGAATAACTTACCCCTTTTT 901 TCGGTTTAATGGGACATAAAAACATCTT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

---

Primer3Batch v1.0