Sequence ID: DB090B06_F1.ab1

Motif: (AGC)4 Motif length: 3 SSR: AGCAGCAGCAGC SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 619 20 55.35 50.00 3.00 2.00 GTAGCAGCACCCTGTTGTAT 156 927 927 5.00 1.00   REVERSE 774 21 54.64 42.86 5.00 1.00 GTGCAGACAACTTCTTTCACT Primer view: 1 ATATCTGAAAACACCAAAAAAAACCAACAATCTTTCAGTCAGCACGGGACTAGCTAGATC 61 GACGGGTTCGACAGAAGGAGAACCCAAATTAACGAGACGGATGGACATTGATTTCTCCTT 121 ACCATCTTCTTCTGAATCGCCGGCATCGGCGACGCCGCGACCTGTCGATCGAGCTTCTGC 181 CAGGCATCGGACATGTCGCAGGCGGACTTGAGGCAAACGGGACACGAGAACCTGAGAGGA 241 AAAAATGTCGCATATATGCTGACACCATGTTCATGGGGTAATTAAGTCTATCTGACCCTG 301 TGTCTGTGTGGATATAGATATACTTACCGTCGATGTGCTCTCATCTCGCGGAGGCATTCC 361 ACGTGGATCATGTGCCCGCACCGCAGCACGTTGATATCTCTCGCCGACTCGAACATGTAC 421 TGTAGCAGAAATCAGATAAAGGGGAAGCCGAAGCAAAAAGCAAAAATTCAATCAGAAACA 481 GGGGAATTCAAATTCTTTGCTGAAGATGATCTCTCTGGAGTCTGAGTCCGGAAGAAAGAT 541 GTTCACCTCGAAGCATATAGGGCAGTTCTGGTGCATTGCCCTATCGACGCAGCGATGGGA 601 ATCCTTCAGCACGAAGCTGTAGCAGCACCCTGTTGTATACACAGGCTCAGTGCAACGATG                      >>>>>>>>>>>>>>>>>>>>                      661 CAGACTTGTTAGCAGCAGCAGCAAAGGATTCAGTAACTTGAGTATAAGTCCATTTGGCAC 721 CAAGAAGGAAGGTTTAGGTACCGCATTTCTCGCAGTGAAAGAAGTTGTCTGCACCGCCGG                                    <<<<<<<<<<<<<<<<<<<<<       781 TTCTGCGAAAGACGGAGTACAATTGTAAACTAGGATTTGGTATTGGCCTATTATGGTAAA 841 TTACAAGGGGTGATATGTCAATGCAGATACAGCACTACTTAGCGCTTGCCTGCATATGCC 901 GCATCTGTCGCAGTGAAACTGGTTCTT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0