Sequence ID: DB081A05_R2.ab1

Motif: (ACAC)5 Motif length: 4 SSR: ACACACACACACACACACAC SSR length: 20 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 366 18 55.47 55.56 4.00 0.00 GGTTAGCACTGACGTGGA 231 925 925 3.00 2.00   REVERSE 596 19 55.39 42.11 4.00 2.00 GAAAACAAAAGCGAAGCTC Primer view: 1 AGGATCCCAAATTCCCAGCACAAGATATAGAGCAGCTACCAAGTTACGAATTCTAAGACT 61 GGATTCAACCTTAGAGTGACGCCAAAACCAAACATTGCATTTCATCGAACAGCTTGCCTA 121 CATCTCAGAGGCATTTAAGCAAACACATTGCATCCCAGGGGATGAGGCTTGACTTACAGC 181 TTTGCGGGATGGGTTGAGCGCTGCCGTCGAGGAAGAGGCGGAGCGACGCAGCCGGCCGTG 241 GTTAGCGCCGTCGAGGGAGAGAGCGGACCGGTGAGAGCTCCGTCGAGGGAGAGACAGAGC 301 GGCGGAGCAGCCGCCTGGGCTTAGCTCTGCCGTCGAGGAAGGAGTGGAGCGACGCAGCCC 361 ACAGGGGTTAGCACTGACGTGGAGGAAGGGAGGAAGCGCAGCCGCAGCCGGCCGGGGTGA         >>>>>>>>>>>>>>>>>>                                     421 GCGCCGACGAGGGAGAGAGCCGACCGCCGGTGTGGCGGCTAGGGTCCCTCCTAGAGAGGG 481 GCAAACACACACACACACACACACACAAACGCCCACAGCCCACAGGCCTCTTCAGCCGCC 541 GTTGCTTGCTGGGCCATGAATCGCGGCCCAAAGTTGGGAGCTTCGCTTTTGTTTTCTGCC                                        <<<<<<<<<<<<<<<<<<<     601 CTATTTGATTTCTTTCTTCCCCTCAATTTTTTTTTTATTTCTTTCTTTCTTCGCTTTCTT 661 TTTTACCAAATGTTTCTGCCGTATATTTGTTTTCTTTTTTCCTTGTACCAAATGTTTTCT 721 GCCTTTTTTTCTTTCTCTGACATCTGTACGCATCTCCGCCTTTTTCCTCGAAAAATAAAA 781 GAAAGATAACCTTTCAAAACATTGCACTTAATTCTAAACCAAACGTAGCATTGGAAAATT 841 AATAGGAGTATACGTTTTTTTCCTATGGCAAGATATTTAGAATAACACTTTAAATATTCC 901 CACGGCCACATTTCATCGAACTGCT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

---

Primer3Batch v1.0