Sequence ID: DB081A05_R2.ab1

Motif: (TTTC)3 Motif length: 4 SSR: TTTCTTTCTTTC SSR length: 12 SSR flanking primers: Orientation Start Len Tm GC% Any 3' Primer Seq Product Size Seq Size Included Size Pair_any Pair_3' 1 FORWARD 607 20 55.91 40.00 2.00 0.00 GATTTCTTTCTTCCCCTCAA 145 925 925 6.00 3.00   REVERSE 751 21 55.20 47.62 4.00 0.00 GCGTACAGATGTCAGAGAAAG Primer view: 1 AGGATCCCAAATTCCCAGCACAAGATATAGAGCAGCTACCAAGTTACGAATTCTAAGACT 61 GGATTCAACCTTAGAGTGACGCCAAAACCAAACATTGCATTTCATCGAACAGCTTGCCTA 121 CATCTCAGAGGCATTTAAGCAAACACATTGCATCCCAGGGGATGAGGCTTGACTTACAGC 181 TTTGCGGGATGGGTTGAGCGCTGCCGTCGAGGAAGAGGCGGAGCGACGCAGCCGGCCGTG 241 GTTAGCGCCGTCGAGGGAGAGAGCGGACCGGTGAGAGCTCCGTCGAGGGAGAGACAGAGC 301 GGCGGAGCAGCCGCCTGGGCTTAGCTCTGCCGTCGAGGAAGGAGTGGAGCGACGCAGCCC 361 ACAGGGGTTAGCACTGACGTGGAGGAAGGGAGGAAGCGCAGCCGCAGCCGGCCGGGGTGA 421 GCGCCGACGAGGGAGAGAGCCGACCGCCGGTGTGGCGGCTAGGGTCCCTCCTAGAGAGGG 481 GCAAACACACACACACACACACACACAAACGCCCACAGCCCACAGGCCTCTTCAGCCGCC 541 GTTGCTTGCTGGGCCATGAATCGCGGCCCAAAGTTGGGAGCTTCGCTTTTGTTTTCTGCC 601 CTATTTGATTTCTTTCTTCCCCTCAATTTTTTTTTTATTTCTTTCTTTCTTCGCTTTCTT          >>>>>>>>>>>>>>>>>>>>                                  661 TTTTACCAAATGTTTCTGCCGTATATTTGTTTTCTTTTTTCCTTGTACCAAATGTTTTCT 721 GCCTTTTTTTCTTTCTCTGACATCTGTACGCATCTCCGCCTTTTTCCTCGAAAAATAAAA             <<<<<<<<<<<<<<<<<<<<<                              781 GAAAGATAACCTTTCAAAACATTGCACTTAATTCTAAACCAAACGTAGCATTGGAAAATT 841 AATAGGAGTATACGTTTTTTTCCTATGGCAAGATATTTAGAATAACACTTTAAATATTCC 901 CACGGCCACATTTCATCGAACTGCT Outer forward primer: >>>>>>>>>> Outer reverse primer: <<<<<<<<<< Oligo primer: ^^^^^^^^^^ Forward SBE/Allele primer: Blue Reverse SBE/allele primer: Brown Target: Red Included region: Green excluded region: Orange

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Primer3Batch v1.0