| Mapping was performed on a set of sixty-five recombinant inbreds derived by single-seed descent (F7) from a cross between the durum wheat cv Messapia (T. turgidum ssp. durum) and the accession MG4343 of T. turgidum ssp. dicoccoides. |
| Ninety-eight primer pairs of wheat microsatellites (WMS) developed for all chromosomes of the A- and B- genomes of hexaploid wheat were used for analysis. Fragment sizes were calculated by Fragment Manager 1.2 (Pharmacia) by comparison with internal size standards. |
| Markers were integrated into the existing RFLP map of durum wheat (see TAG-97-721, and Map_Data : T. turgidum, Messapia x dicoccoides), using the 'PLACE' and 'TRY' commands of the program MAPMAKER 2.0. |
| Eighty-one out of ninety-six (84.4%) microsatellites used for analysis detected polymorphisms, and eight-five loci amplified by seventy-nine primer pairs could be placed on the RFLP map of durum wheat. |