| An RFLP linkage map was constructed using 120 F2 plants from a cross between Ko A (a Japanese two-rowed malting barley) and Mokusekko 3 (a Chinese six-rowed barley landrace). |
| Polymorphism was detected using BamHI, BglII, DraI, EcoRV, HindIII and XbaI restriction enzymes. |
| 222 loci were mapped with an average distance of 6.5 cm between markers for a total of 1389 cM, and included 147 genomic DNA RFLPs, 73 cDNA RFLPs, one isozyme (Est1) and one morphological (vrs1) marker. Only three gap regions exceeding 25 cM were detected. |
| 117 RFLP loci were mapped using 91 previously reported probes and 103 loci were mapped using new JBG or JBC probes. |