| The Q gene is thought to be involved in gene regulation and has effects on the free-threshing character of the spike and influences agronomic characters such as glume keeledness, rachis toughness, spike length, spike type and culm height. Several studies have mapped the Q gene to the long arm of chromosome 5A, and recently, Endo and Gill mapped Q to a cytological submicroscopic deletion interval on 5AL. See GrainGenes reference JHT-87-295. |
| In this study, AFLP and mRNA differential display were performed on deletion lines 5AL-23 and 5AL-7. Clones that were present in the interval between breakpoints 5AL-7 and 5AL-23 (which flanked the Q locus) were then used as RFLP probes to produce a high resolution map using a population of 190 F2s from Chinese Spring crossed with Chinese Spring with Triticum dicoccoides 5A chromosomes substituted into the genome (CS-TDIC DS5A). Markers were identified as close as 0.7 cM to the Q gene. This population was also scored for spike morphology. |
| The linkage map was produced using Mapmaker (v. 2.0) using the Kosambi mapping function and an LOD of 3.00. |
