GrainGenes Marker Report: BCD266a

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Locus	BCD266a
Type	RFLP
Chromosome Arm	1AS
Map	Ta-Anderson-1A 35 Tt-Gill-2D 357.2
	[ Show Nearby Loci ]
Map Data	T.tauschii
Probe	BCD266
Homology	AAD11455.2 e-value: 1.3 (U82549) sodium/potassium-transporting ATPase alpha-1 subunit [Oreochromis mossambicus] AAD11455.2 e-value: 2.1 (U82549) sodium/potassium-transporting ATPase alpha-1 subunit [Oreochromis mossambicus]
Possible Orthologs	BCD266 [ Show all 6 ]
Probe for Locus	BCD266
Locus	Xbcd266-2 [ Show all 7 ]
Synonym	Synonym HAR35_15015   HAR35_15016   HAR35_33212
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library Bar.LambZAPII [c] Bar.LambZAPII
Type	cDNA
Sequence	BE438697 BE438789 BCD266_WHE1B0101 BCD266_WHE1B0102
Polymorphism	BCD266 EcoRI
Linkage Group	[b 2L]
Insert Enzyme	EcoRI
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
Insert Size	1.5
PCR size	1.5
Clone Vector	pBluSK pBluSK- BS-SK- lambda_Zap pBS
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse m13reverse 5'AACAGCTATGACCATG m13forward 5'GTAAAACGACGGCCACT
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	BCD266 BROWS autoradiogram
Data Source	Sorrells, Mark E. 96.07 ATMI 92.09 Gill, Bikram S. 92.09