GrainGenes Marker Report: Bmac0031

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Locus	Bmac0031
Type	Microsatellite
Synonym	Synonym Bmac31
Chromosome	7H
Map	Barley, B73xCPI-7H 87 [ Show all 9 ]
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 9 ]
Species	Hordeum vulgare
Probe	Bmac0031 Bmac31
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Probe for Locus	Bmac31
Locus	Bmac0031 Bmac31 Bmac31a Bmac31b
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)28 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.37
Type	PCR SSR
PCR primers	5' AGAGAAAGAGAAATGTCACCA 3' 5' ATACATCCATGTGAGGGC 3' AGAGAAAGAGAAATGTCACCA ATACATCCATGTGAGGGC
SSR size	175
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare
Probe	Bmac0031
Locus	Bmac0031
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391.
Type	SSR
PCR primers	AGAGAAAGAGAAATGTCACCA ATACATCCATGTGAGGGC Forward AGAGAAAGAGAAATGTCACCA Reverse ATACATCCATGTGAGGGC