GrainGenes Marker Report: Bmag0381

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Locus	Bmag0381
Type	Microsatellite
Synonym	Synonym Bmag381
Chromosome	2H
Map	Barley, B73xCPI-2H 75.7 [ Show all 8 ]
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 8 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmag0381 Bmag381
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (CT)7(ATCT)6 (CT)14 Developing laboratory = SCRI (R. Waugh)
Probe for Locus	Bmag381
Locus	Bmag0381 Bmag381
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (CT)7(ATCT)6(CT)14 Quality = 2: Single product, relatively strong stutter bands Null in H. spontaneum Canada Park.
Type	PCR SSR
PCR primers	5' TTTTATTATTGCATCTAGGGC 3' 5' TATCAAGATCATGACGTCTCA 3'
SSR size	141
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare
Probe	Bmag0381
Locus	Bmag0381
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Type	SSR
PCR primers	TTTTATTATTGCATCTAGGGC TATCAAGATCATGACGTCTCA