GrainGenes Marker Report: CFA2149

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Probe	CFA2149
Locus	Xcfa2149-4B Xcfa2149-5A
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TG)20 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' CTTGGAGCTCGGGTAGTAGC 3' Reverse 5' AAGGCAGCTCAATCGGAGTA 3'
SSR size	231
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	4B
Source Species	Triticum urartu
Data Source	Sourdille, Pierre
Locus for Probe	Xcfa2149-4B
Map	Chinese_Spring_Deletion_SSR_4B 0.93 Ta-Courtot/CS-GPW-4B 94.4
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Breakpoint Interval	4BL1-0.86-1.00
Map Data	Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR
Probe	CFA2149
Locus for Probe	Xcfa2149-5A
Map	Kofa/UC1113-5A 205.9
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Map Data	Durum wheat, Kofa x UC1113
Probe	CFA2149