|Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.|
|Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.|
|Insert contains repeat (GA)11.|
|5' CAGGTGTCTAGTGGGTGCCT 3'|
5' TTACATACCAAGGAGCAATCCC 3'
|The 'touchdown' PCR comprised 48 cycles of 94oC for 1 min denaturing and 72oC for 1 min extension. Annealing (30 s) temperatures were progressively decreased by 1oC every second cycle from 64oC to 55oC. Annealing conditions of 1 min at 55oC were maintained during the final 30 cycles. The reaction ended with a 5-min extension at 72oC.|
|Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.|