GrainGenes Marker Report: WMC85

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Locus	wmc85
Map	Physical-3B 60.42
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Map Data	3B Physical
Probe	WMC85
Locus	Xwmc85-1B Xwmc85a Xwmc85b Xwmc85 Xwmc85.2
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6g9 Donor: Devaux, Florimond Desprez AGR119. Trimmed to insert at 3' end(CA)8 153 to 168, (CA)18 184 to 219. Products a little weak at 61C, try lower anneal?
Type	PCR SSR
PCR primers	Forward ggAgTAAgAgAAAcATgccgAA at 27 length 22 bases, Tm 62 Reverse gTgcATgcATgAgAATAggAAc at 233 length 22 bases, Tm 61
SSR size	228 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC85
Source Species	Triticum aestivum
Data Source	Somers, Daryl J. 2004.06 Isaac, Peter G. 2004.06
Locus for Probe	Xwmc85
Type	Microsatellite
Chromosome	1D
Map	Wheat-Composite2004-1D 9
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Map Data	Wheat, Composite, 2004
Probe	WMC85
Locus for Probe	Xwmc85-1B
Map	Kofa/UC1113-1B 40.2 Ta-Synthetic/Opata-GPW-1B 11.3
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Map Data	Durum wheat, Kofa x UC1113 Wheat, Synthetic x Opata, GPW
Probe	WMC85
Locus for Probe	Xwmc85.2
Type	Microsatellite
Chromosome	3B
Map	Wheat-Composite2004-3B 35
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Map Data	Wheat, Composite, 2004
Probe	WMC85
Locus for Probe	Xwmc85a
Type	Microsatellite
Chromosome	1B
Map	Wheat-Composite2004-1B 22
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Map Data	Wheat, Composite, 2004
Probe	WMC85
Locus for Probe	Xwmc85b
Type	Microsatellite
Chromosome	1B
Map	Wheat-Composite2004-1B 69
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC85