GrainGenes Marker Report: Xbarc232-5D

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Locus	Xbarc232-5D
Type	Microsatellite
Chromosome	5D
Map	Ta-SSR-2004-5D 81.4
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Map Data	Wheat, Consensus SSR, 2004
Probe	BARC232
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Probe for Locus	BARC232
Locus	Xbarc232-5B Xbarc232 Xbarc232-5A Xbarc232-5D
General Remarks	Contains motif : (CT)18 BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type	PCR SSR
PCR primers	5' CGCATCCAACCATCCCCACCCAACA 3' 5' CGCAGTAGATCCACCACCCCGCCAGA 3'
Amplification Conditions	Annealing temperature = 65
Sequence	BV211860
Source Species	Triticum aestivum
Data Source	Ward, Rick