GrainGenes Marker Report: Xbcd102-2D

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Locus	Xbcd102-2D
Type	RFLP
Chromosome	2D
Chromosome Arm	2DS
Map	Ta-Synthetic/Opata-2D 40.5 [ Show all 7 ]
	[ Show Nearby Loci ]
Map Data	Wheat, Synthetic x Opata  [ GBrowser ] [ Show all 7 ]
Probe	BCD102
Homology	METL_ORYSA e-value: 1e-59 S-ADENOSYLMETHIONINE SYNTHETASE 2 (METHIONINE ADENOSYLTRANSFERASE 2) (ADOMET SYNTHETASE 2) AAF46202.1 e-value: 4.8 (AE003438) CG4532 gene product [Drosophila melanogaster] T50301 e-value: 5.0 probable transcription initiation factor IIE alpha subunit [imported] - fission yeast (Schizosaccharomyces pombe) (fragment) subunit [Schizosaccharomyces pombe]
Possible Orthologs	BCD102 BCD102B Xbcd102-6B
Probe for Locus	BCD102
Locus	Xbcd102-2D [ Show all 10 ]
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library Bar.LambZAPII [c]
Type	cDNA
Sequence	BE438640 BE438719 BE438811 BCD102_WHE1B0121 BCD102_WHE1B0122
Polymorphism	BCD102 EcoRI
Linkage Group	3S, 6L
Insert Enzyme	EcoRI
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
Insert Size	1.8
PCR size	1.8
Clone Vector	pBluSK pBluSK-
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	BCD102 SO autoradiogram
Data Source	Sorrells, Mark E. 96.07 ATMI 92.09