GrainGenes Marker Report: Xbcd1212

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Locus	Xbcd1212
Type	RFLP
Map	Oat-KxO-28 49.8
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Map Data	Oat, KxO
Probe	BCD1212
Homology	T49510 e-value: 0.82 fibroin-3 related protein [imported] - Neurospora crassa
Image	BCD1212 KO autoradiogram
Probe for Locus	BCD1212
Locus	Xbcd1212 bcd1212
Synonym	Synonym HAR35_1275
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library
Type	cDNA
Sequence	BE438936 BCD1212_WHE04E06.s1
Polymorphism	BCD1212 DraI
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
PCR size	1.2
Clone Vector	pBluSK
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	BCD1212 KO autoradiogram
Data Source	Sorrells, Mark E. 96.07