GrainGenes Marker Report: Xbcd1428

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Locus	Xbcd1428
Type	RFLP
Chromosome	3A
Map	Avena-Sorrells-B 51.4 Wheat-Composite2004-3A 37
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Map Data	Diploid Oat, Cornell2 Wheat, Composite, 2004
Probe	BCD1428
Possible Orthologs	Xbcd1428-3A
Probe for Locus	BCD1428
Locus	Xbcd1428-3A Xbcd1428 Xbcd1428a
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library
Type	cDNA
Polymorphism	BCD1428 EcoRV
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
PCR size	1.4
Clone Vector	pBluSK
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E.
Clone Authority	Sorrells, Mark E.
Image	BCD1428 SO autoradiogram
Data Source	Sorrells, Mark E. 96.07