GrainGenes Marker Report: Xbcd1510-6A

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Locus	Xbcd1510-6A
Type	RFLP
Chromosome	6A
Chromosome Arm	6AL
Map	Ta-Synthetic/Opata-6A 69 Ta-Synthetic/Opata-BARC-6A 106.5 Ta-Synthetic/Opata-GPW-6A 131.6
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Map Data	Wheat, Synthetic x Opata  [ GBrowser ] Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, GPW
Probe	BCD1510
Image	BCD1510 SO autoradiogram
Possible Orthologs	Xbcd1510-6 Xbcd1510-6D
Probe for Locus	BCD1510
Locus	Xbcd1510-6 Xbcd1510-6D Xbcd1510-6A Xbcd1510b Xbcd1510a
Synonym	Other name BCD880
Related Probe	BCD880
	Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library
Type	cDNA
Polymorphism	BCD1510 EcoRI   BCD1510 EcoRV   BCD1510 HindIII   BCD1510 XbaI
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
PCR size	0.9
Clone Vector	pBluSK
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E.
Clone Authority	Sorrells, Mark E.
Image	BCD1510 SO autoradiogram
Data Source	Sorrells, Mark E. 96.07