GrainGenes Marker Report: Xbcd1562-1

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Locus	Xbcd1562-1
Type	RFLP
Chromosome Arm	1L
Map	Triticeae-Sorrells-1 131
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Map Data	Triticeae consensus
Probe	BCD1562
Possible Orthologs	Xbcd1562 Xbcd1562-1B
Probe for Locus	BCD1562
Locus	Xbcd1562-1 [ Show all 6 ]
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library
Type	cDNA
Polymorphism	BCD1562 EcoRI   BCD1562 HindIII   BCD1562 EcoRV   BCD1562 DraI   BCD1562 XbaI
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
PCR size	1.2
Clone Vector	pBluSK
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	BCD1562 KO autoradiogram BCD1562 SO autoradiogram
Data Source	Sorrells, Mark E. 96.07