GrainGenes Marker Report: Xbcd880-6B

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Locus	Xbcd880-6B
Type	RFLP
Map	Tturgidum-Du-6B 38.7
	[ Show Nearby Loci ]
Map Data	T. turgidum, durum x dicoccoides
Probe	BCD880
Homology	T03766 e-value: 1e-28 probable glutathione reductase (NADPH) (EC 1.6.4.2) - rice
Probe for Locus	BCD880
Locus	Xbcd880-6A [ Show all 7 ]
Synonym	Please see BCD1510 Synonym HAR35_15570
Related Probe	BCD1510
	Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library Based on surveys and mapping in Chinese Spring aneuploids and the wheat Synthetic x Opata population, this probe appears to be identical to BCD1510, and should be referred to by that name. (Clare Nelson, August 1995)
Type	cDNA
Sequence	BE438921 BCD880_WHE04A06.s1
Polymorphism	BCD880 EcoRV
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
PCR size	0.8
Clone Vector	pBluSK
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	BCD880 KO autoradiogram BCD880 SO autoradiogram
Data Source	Sorrells, Mark E. 96.07