GrainGenes Marker Report: Xcfa2155-5A

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Locus	Xcfa2155-5A
Type	Microsatellite
Chromosome	5A
Map	Chinese_Spring_Deletion_SSR_5A 0.825 Ta-Courtot/CS-GPW-5A 188.5 Ta-SSR-2004-5A 103.1
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Breakpoint Interval	5AL17-0.78-0.87
Map Data	Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR
Probe	CFA2155
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Probe for Locus	CFA2155
Locus	Xcfa2155-5A
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = A Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TG)16 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TTTGTTACAACCCAGGGGG 3' Reverse 5' TTGTGTGGCGAAAGAAACAG 3'
SSR size	213
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5A
Source Species	Triticum urartu
Data Source	Sourdille, Pierre