|Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.|
|Quality Score = B|
|Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)|
|Amplifies motif: (TC)16|
|Regularity of motif is perfect|
|Forward 5' TTGATCCTTGATGGGAGGAG 3'|
Reverse 5' CATCATTGTGTTTACGTTCTTTCA 3'
|5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees|