GrainGenes Marker Report: Xcfd20-1B

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Locus	Xcfd20-1B
Map	Conan/Reeder-1B 53.0508 Ta-Courtot/CS-GPW-1B 59.4
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Map Data	Wheat, Conan x Reeder Wheat, Courtot x Chinese Spring, GPW
Probe	CFD20
Probe for Locus	CFD20
Locus	Xcfd20-1B Xcfd20 Xcfd20-5B Xcfd20-7A
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GGAA)3(CTAC)3 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' TGATGGGAAGGTAATGGGAG 3' Reverse 5' ATCCAGTTCTCGTCCAAAGC 3'
SSR size	296
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1B
Source Species	Aegilops tauschii
Data Source	Sourdille, Pierre