|Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.|
|Quality Score = C|
|Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)|
|Amplifies motif: (GGAA)3(CTAC)3|
|Regularity of motif is imperfect|
|Forward 5' TGATGGGAAGGTAATGGGAG 3'|
Reverse 5' ATCCAGTTCTCGTCCAAAGC 3'
|5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees|