GrainGenes Marker Report: Xgwm261

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Locus	Xgwm261
Type	Microsatellite
Chromosome	2D
Map	Ta-NxW-2D 37.7 Wheat-Composite2004-2D 22
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Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMS261
Probe for Locus	WMS261
Locus	Xgwm261-2D Xgwm261 Rht8
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023. Korzun V et al. (1997) Application of microsatellite markers to distinguish inter-varietal chromosome substitution lines of wheat (Triticum aestivum L.). Euphytica 95:149-155.
General Remarks	Insert contains repeat (CT)21 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' CTCCCTGTACGCCTAAGGC 3' 5' CTCGCGCTACTAGCCATTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS261
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring
Data Source	Roeder, Marion S. 98.09.15 Korzun, Victor N. 97.10