GrainGenes Marker Report: Xgwm473-2A

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Locus	Xgwm473-2A
Type	Microsatellite
Chromosome	2A
Map	Chinese_Spring_Deletion_SSR_2A -0.39 [ Show all 6 ]
	[ Show Nearby Loci ]
Breakpoint Interval	C-2AS5-0.78
Map Data	Wheat, Conan x Reeder [ Show all 6 ]
Probe	WMS473
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-2A, the intervals most likely to contain locus are Xbcd152 - Xgwm71.2 or Xgwm71.2 - Xbcd543
Probe for Locus	WMS473
Locus	Xgwm473-2A Xgwm473 Xgwm473-7D Xgwm473-7A
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GT)14(TTGG)(GT)8 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TCATACGGGTATGGTTGGAC 3' 5' CACCCCCTTGTTGGTCAC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS473
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring
Data Source	Roeder, Marion S. 98.09.15