GrainGenes Marker Report: Xpsr149-6R

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Locus	Xpsr149-6R
Type	RFLP
Chromosome	6R
Map	Secale-PxP-2004-6R 47.3
	[ Show Nearby Loci ]
Map Data	Rye, P87 x P105, 2004
Species	Secale cereale
Probe	PSR149
Reference	Khlestkina EK et al. (2004) Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags Theoretical and Applied Genetics 109:725-732.
Probe for Locus	PSR149
Locus	Xpsr149-6A [ Show all 8 ]
Synonym	Synonym HAR35_15356
General Remarks	Polymorphism scores are given as the percent polymorphic pairwise comparisons, followed by the number of alleles found and number of varieties tested in parentheses.
Type	cDNA
Sequence	PSR149
Copy Number	1
Background	2
Cross hybridizes to	Secale cereale good Hordeum vulgare good
Polymorphism	PSR149 H EcoRI 73.3 (3, 6) [ Show all 11 ]
Gel	PSR149 Gale
Linkage Group	6AL [ Show all 6 ]
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring
Source Tissue	Leaf
Insert Size	0.4
Clone Vector	pUC18
Vector Enzyme	SmaI
Excision Enzyme	HindIII EcoRI
Vector PCR primers	5'-d(GTTTTCCCAGTCACGAC)-3' 5'-d(CAGGAAACAGCTATGAC)-3'
Clone Location	Gale, Michael D.
Clone Authority	Chao, Shiaoman
Data Source	Gale, Michael D. 97.09 Gill, Bikram S. 92.09