GrainGenes Marker Report: Xwmc179.1-2A

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Locus	Xwmc179.1-2A
Map	Louise/Penawawa-2A 24.6
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Map Data	Wheat, Louise x Penawawa
Probe	WMC179
Probe for Locus	WMC179
Locus	Xwmc179.1-1D [ Show all 13 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9h5 Donor: Bernard, Clermont-Ferrand Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 132 bases located on 2A, 136 bases located on 1D, 142 bases located on 7A, 198 bases located on 6A, 237 bases located on 2B, 261 bases located on 3D, 365 bases located on 6A Trimmed to insert at both ends. (GA)17 415 to 448. Bases 1 to 507 show homology to 15h1 bases 1 to 507 (507 in longest run, maxScore = 414). 9h5 is a duplicate of 15h1. 9h5 has priority.
Type	PCR SSR
PCR primers	Forward cATggTggccATgAgTggAggT at 301 length 22 bases, Tm 70 Reverse cATgATcTTgcgTgTgcgTAgg at 480 length 22 bases, Tm 68
SSR size	201 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC179
Source Species	Triticum aestivum
Data Source	Somers, Daryl J. 2004.06 Isaac, Peter G. 2004.06