GrainGenes Marker Report: Xwmc416

[ Printable Version ]  [ Submit comment/correction ]

Locus	Xwmc416
Type	Microsatellite
Chromosome	6D
Map	Wheat-Composite2004-6D 77
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC416
Probe for Locus	WMC416
Locus	Xwmc416-6D Xwmc416 Xwmc416-1B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 23a3 Donor: Romestant, RAGT Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 223 bases located on 6D Trimmed to Ssp1 at both ends of insert. (CA)11 239 to 260, (CA)11 263 to 284, (CA)8 287 to 302.
Type	PCR SSR
PCR primers	Forward AgcccTTTcTAccgTgTTTcTT at 169 length 22 bases, Tm 62 Reverse TATggTcgATggAcTgTcccTA at 367 length 22 bases, Tm 63
SSR size	220 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC416
Source Species	Triticum aestivum
Data Source	Somers, Daryl J. 2004.06 Isaac, Peter G. 2004.06