GrainGenes Marker Report: cdo216b

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Locus	cdo216b
Type	RFLP
Map	Oat-OxT-20 0
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Map Data	Oat, OxT
Probe	CDO216
Homology	AAB92527.1 e-value: 9.4 (AF033595) maturase [Paeonia sinjiangensis]
Probe for Locus	CDO216
Locus	cdo216a [ Show all 8 ]
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Uni-ZAP XR library
Type	cDNA
Sequence	BE439267 CDO216_WHE04D07.s1 CDO216_WHE04F01.s1
Polymorphism	CDO216 EcoRI   CDO216 EcoRV   CDO216 DraI
Source Species	Avena sativa
Source Germplasm	Brooks CIav9260
Source Tissue	etiolated leaf
PCR size	1
Clone Vector	pBluSK
Excision Enzyme	EcoRI XhoI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	CDO216 KO autoradiogram
Data Source	Sorrells, Mark E. 96.07