GrainGenes Marker Report: CDO0270

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Locus	CDO0270
Type	RFLP
Map	Avena-Wise-E 63.8
	[ Show Nearby Loci ]
Map Data	Diploid Oat, Iowa St.
Probe	CDO270
Homology	NP_070556.1 e-value: 2.2 galactosyltransferase [Archaeoglobus fulgidus] fulgidus
Possible Orthologs	[ Hide all but 1 of 7 ] CDO270 Xcdo270 Xcdo270-6 Xcdo270-6A Xcdo270-6D Xcdo270A Xcdo270B
Probe for Locus	CDO270
Locus	Xcdo270-6 [ Show all 15 ]
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Uni-ZAP XR library
Type	cDNA
Sequence	BE439259 CDO270_WHE04F02.s1 CDO270-5 CDO270-3
Polymorphism	CDO270 EcoRI   CDO270 EcoRV   CDO270 DraI   CDO270 XbaI   CDO270 HindIII
Source Species	Avena sativa
Source Germplasm	Brooks CIav9260
Source Tissue	etiolated leaf
PCR size	1.5
Clone Vector	pBluSK
Excision Enzyme	EcoRI XhoI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	CDO270 KO autoradiogram CDO270 SO autoradiogram
Data Source	Sorrells, Mark E. 96.07