GrainGenes Marker Report: EBmac0602

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Locus	EBmac0602
Type	Microsatellite
Synonym	Synonym EBmac602
Chromosome	6H
Map	Barley, B73xCPI-6H 54.6 [ Show all 9 ]
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 9 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	Bmac602 EBmac0602 EBmac602
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	[ Hide all but 1 of 7 ] Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Steptoe x Morex Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Steptoe x Morex Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Steptoe x Morex, Lina x H. spontaneum Insert contains repeat (AC)9AT(AC)7 (AG)9 Polymorphic Information Content (PIC) = 0.62 Developing laboratory = SCRI (R. Waugh)
Probe for Locus	Bmac602
Locus	EBmac0602
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Type	SSR
PCR primers	GATTGGAGCTTCGGATCAC CCGTCTAGGGAGAGGTTCTC
Probe for Locus	EBmac602
Locus	EBmac0602 EBmac602
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)9AT(AC)7(AG)9 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.62
Type	PCR SSR
PCR primers	5' GATTGGAGCTTCGGATCAC 3' 5' CCGTCTAGGGAGAGGTTCTC 3'
SSR size	205
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare
Probe	EBmac0602
Locus	EBmac0602
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391.
Type	SSR
PCR primers	GATTGGAGCTTCGGATCAC CCGTCTAGGGAGAGGTTCTC