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GrainGenes Marker Report: GBM1253

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Locus
GBM1253
Type
Microsatellite
Chromosome
3H
Map
Hordeum-Consensus2007-SSR-3H66.38
Hv-Integrated2009-3H76.4
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2007, SSR
Barley, Integrated, Marcel 2009
Species
Hordeum vulgare
Probe
GBM1253
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceVarshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark
[ Hide all but 1 of 6 ]
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Oregon Wolfe Barleys
Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : OWBrecessive x OWBdominant
Insert contains repeat (AG)9
Polymorphic Information Content (PIC) = 0.65
Developing laboratory = IPK (A. Graner)



Probe
GBM1253
Locus
GBM1253
GBM1253-3H
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceVarshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks
Clone HI01K11T
Type
SSR
PCR primers
CCACAAACCCCGTAACCTC
CTCGTTGGAGAGGAGGGAG
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
BQ462670
DNA Library
IPK-HI
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
Data Source
Stein, Nils2006.10



Locus for Probe
GBM1253-3H
Type
Microsatellite
Map
Hv-Consensus2006-Stein-3H83.6
Hv-DxR-Stein-3H84.3
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Stein
Barley, OWB, Stein2006
Probe
GBM1253

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