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GrainGenes Marker Report: GBS0823-3H

Locus
GBS0823-3H
Type
SNP
Map
Hv-Consensus2006-Stein-3H68.9
Hv-SxM-Stein-3H50.83
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Stein
Barley, SxM, Stein2006
Probe
GBS823



Probe for Locus
GBS823
Locus
GBS0823
GBS0823-3H
Reference
ReferenceStein N et al. (2007) A 1000 loci transcript map of the barley genome - new anchoring points for integrative grass genomics Theoretical and Applied Genetics 114:823-839.
General Remarks
Clone HZ47G23r
PCR primers
AAGTGGCTCGGCACCTTC
GACGACGCGTTTGACCC
Amplification Conditions
5 min at 94 deg C; 50 cycles with 30 sec at 94 deg C, 1 min at 65 deg C (touchdown of 1 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 40 cycles), 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
CA023797
DNA Library
IPK-HZ
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
Data Source
Stein, Nils2006.10