GrainGenes Marker Report: Xbcd1495-6B

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Locus	Xbcd1495-6B
Type	RFLP
Chromosome	6B
Chromosome Arm	6BS
Map	Ta-Synthetic/Opata-6B -2.5 Ta-Synthetic/Opata-BARC-6B 36.4 Ta-Synthetic/Opata-DR-6B -3.6 Ta-Synthetic/Opata-GPW-6B 46.6 Ta-Synthetic/Opata-SSR-6B 57
	[ Show Nearby Loci ]
Map Data	Wheat, Synthetic x Opata  [ GBrowser ] Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, DR Genes Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR
Probe	BCD1495
Homology	AF346305_1 e-value: 4e-47 (AF346305) S-adenosyl-L-methionine synthetase [Elaeagnus umbellata] METL_CATRO e-value: 2e-60 S-ADENOSYLMETHIONINE SYNTHETASE 2 (METHIONINE ADENOSYLTRANSFERASE 2) (ADOMET SYNTHETASE 2) METL_CATRO e-value: 2e-37 S-ADENOSYLMETHIONINE SYNTHETASE 2 (METHIONINE ADENOSYLTRANSFERASE 2) (ADOMET SYNTHETASE 2) METL_CATRO e-value: 2e-49 S-ADENOSYLMETHIONINE SYNTHETASE 2 (METHIONINE ADENOSYLTRANSFERASE 2) (ADOMET SYNTHETASE 2)
Possible Orthologs	Xbcd1495
Probe for Locus	BCD1495
Locus	[ Hide all but 1 of 6 ] Xbcd1495-6B bcd1495a bcd1495b Xbcd1495 Xbcd1495-1B bcd1495
Related Probe	CDO1421
	Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library
Type	cDNA
Sequence	BE438821 [ Show all 8 ]
Polymorphism	BCD1495 EcoRI   BCD1495 EcoRV   BCD1495 DraI
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
PCR size	1.3
Clone Vector	pBluSK
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	BCD1495 KO autoradiogram BCD1495 SO autoradiogram
Data Source	Sorrells, Mark E. 96.07