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GrainGenes Marker Report: Xbcd221

Locus
Xbcd221
Type
RFLP
Chromosome
5B
5R
Chromosome Arm
5RL
Map
Avena-Sorrells-F52.3
Secale-ExR-591.8
Wheat-Composite2004-5B117
[ Show Nearby Loci ]
Map Data
Diploid Oat, Cornell2
Rye, ExR
Wheat, Composite, 2004
Probe
BCD221
Possible Orthologs
Xcnl.BCD221A
Xcnl.BCD221B



Probe for Locus
BCD221
Locus
[ Hide all but 1 of 15 ]
Xbcd221-5B
BCD221
Xcnl.BCD221B
Xcnl.BCD221A
Xbcd221
BCD221B
BCD221A
Xbcd221-2B
Xbcd221-4B
Xbcd221e
Xbcd221c
Xbcd221a
Xbcd221b
BCD221(A)
BCD221(C)
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Linkage Group
[b 6, 2S]
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.9
PCR size
0.9
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07
ATMI92.09