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GrainGenes Probe Report: AM3

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Probe
AM3  [ Marker Report ]
Locus
AM3
Reference
ReferenceFu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038.
ReferenceLi CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks
Insert contains repeat (AG)35
Polymorphic in Kanota/Ogle.
Among 20 oat cultivars, 5 alleles were found, polymorphic information content (PIC) = 0.73.
In 369 Avena sterilis accessions there were 24 alleles of size 144-378 bp, PIC = 0.93.
Type
Genomic
PCR
SSR
Microsatellite
PCR primers
5'CTG GTC ATC CTC GCC GTT CA
5'CAT TTA GCC AGG TTG CCA GGT C
SSR size
280
Amplification Conditions
Average melting temperature (degrees C) = 51
Source Species
Avena sativa
Source Germplasm
Ogle CIav9401
Data Source
Scoles, Graham J. 
Fu, Yong-Bi2007.04

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