GrainGenes Probe Report: BCD1194

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Probe	BCD1194  [ Marker Report ]
Locus	Xbcd1194
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library
Type	cDNA
Sequence	BE438621 BE438753 BCD1194_WHE1H0041 BCD1194_WHE1H0042
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
PCR size	0.9
Clone Vector	pBluSK
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Data Source	Sorrells, Mark E. 96.07