GrainGenes Probe Report: BCD1261

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Probe	BCD1261  [ Marker Report ]
Locus	Xbcd1261-1 [ Show all 10 ]
Synonym	Synonym HAR35_32749
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library
Type	cDNA
Sequence	BE438635 [ Show all 6 ]
Polymorphism	BCD1261 DraI   BCD1261 XbaI
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
PCR size	0.8
Clone Vector	pBluSK
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	BCD1261 KO autoradiogram BCD1261 SO autoradiogram
Data Source	Sorrells, Mark E. 96.07