GrainGenes Probe Report: BCD1262

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Probe	BCD1262  [ Marker Report ]
Locus	Xbcd1262-4B Xbcd1262 BCD1262 Xbcd1262-4A Xbcd1262-4D
Synonym	Synonym HAR35_15305
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library Bar.LambZAPII [c] Bar.LambZAPII
Type	cDNA
Sequence	BE438882 BCD1262_WHE03G02.r1
Polymorphism	BCD1262 EcoRI   BCD1262 XbaI
Linkage Group	4L
Insert Enzyme	EcoRI
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
Insert Size	1
PCR size	1
Clone Vector	pBluSK pBluSK- pGEM4
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	BCD1262 BROWS autoradiogram BCD1262 SO autoradiogram
Data Source	Sorrells, Mark E. 96.07 ATMI 92.09