GrainGenes Probe Report: BCD1319

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Probe	BCD1319  [ Marker Report ]
Locus	Xbcd1319-6D Xbcd1319
Synonym	Synonym HAR35_15384   HAR35_2180   HAR35_38577
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library
Type	cDNA
Sequence	BE438976 BCD1319_WHE05C04.s1
Polymorphism	BCD1319 EcoRV   BCD1319 EcoRI   BCD1319 HindIII   BCD1319 DraI
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
PCR size	1.4
Clone Vector	pBluSK
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	BCD1319 SO autoradiogram
Data Source	Sorrells, Mark E. 96.07