GrainGenes Probe Report: BCD1434

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Probe	BCD1434  [ Marker Report ]
Locus	BCD1434 [ Show all 16 ]
Synonym	Synonym HAR35_16734   HAR35_16735   HAR35_42959
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library Bar.LambZAPII [c] Bar.LambZAPII
Type	cDNA
Sequence	BE438866 [ Show all 6 ]
Polymorphism	BCD1434 EcoRI   BCD1434 XbaI   BCD1434 EcoRV   BCD1434 HindIII
Linkage Group	1S
Insert Enzyme	EcoRI
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
Insert Size	1.2
PCR size	1.2
Clone Vector	pBluSK pBluSK- BS-SK- lambda_Zap pBS
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse m13reverse 5'AACAGCTATGACCATG m13forward 5'GTAAAACGACGGCCACT
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	BCD1434 BROWS autoradiogram BCD1434 SO autoradiogram pBCD1434
Data Source	Sorrells, Mark E. 96.07 ATMI 92.09 Gill, Bikram S. 92.09