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GrainGenes Probe Report: BCD1502

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Probe
BCD1502  [ Marker Report ]
Locus
Xbcd1502
BCD1502
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438997
BE439026
BCD1502_WHE05A11.s1
Polymorphism
BCD1502 EcoRI 
BCD1502 EcoRV 
BCD1502 DraI 
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
1.1
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1502 KO autoradiogram
Data Source
Sorrells, Mark E.96.07

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