GrainGenes Probe Report: BCD1819

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Probe	BCD1819  [ Marker Report ]
Locus	Xbcd1819A Xbcd1819B bcd1819a bcd1819b
Synonym	Synonym HAR35_1008   HAR35_15268   HAR35_18078
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library
Type	cDNA
Sequence	BE439005 BE439032 BCD1819_WHE05C11.s1
Polymorphism	BCD1819 EcoRV
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
PCR size	1.7
Clone Vector	pBluSK
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	BCD1819 KO autoradiogram
Data Source	Sorrells, Mark E. 96.07