GrainGenes Probe Report: BCD421

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Probe	BCD421  [ Marker Report ]
Locus	Xbcd421A [ Show all 8 ]
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Lambda ZAP II library Bar.LambZAPII [c]
Type	cDNA
Sequence	BE438611 BE438749 BCD421_WHE1B0041 BCD421_WHE1B0042
Polymorphism	BCD421 DraI
Linkage Group	[b 1S]
Insert Enzyme	EcoRI
Source Species	Hordeum vulgare
Source Germplasm	Willis
Source Tissue	etiolated leaf
Insert Size	1.6
PCR size	1.6
Clone Vector	pBluSK pBluSK-
Excision Enzyme	EcoRI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	BCD421 KO autoradiogram
Data Source	Sorrells, Mark E. 96.07 ATMI 92.09