GrainGenes Probe Report: Bmag877

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Probe	Bmag877  [ Marker Report ]
Locus	Bmag877 Bmag0877
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (GA)15 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.77
Type	PCR SSR
PCR primers	5' AAAGCTCATGGTAGATCAAGA 3' 5' TAGTTTTCCCAAAAGCTTCTA 3'
SSR size	153
Amplification Conditions	3 min 94C, 45 cycles of 1 min @94C, 1 min @55C, 2 min @72C, final extension of 10 min @72C. 25 microlitre reactions contained 125 nM of each primer.
Source Species	Hordeum vulgare