GrainGenes Probe Report: CDO1081

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Probe	CDO1081  [ Marker Report ]
Locus	CDO1081a Xcdo1081 cdo1081b Xcdo1081-4D CDO1081
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Uni-ZAP XR library
Type	cDNA
Sequence	AA231699 AA231841
Polymorphism	CDO1081 EcoRI   CDO1081 EcoRV   CDO1081 XbaI   CDO1081 DraI
Source Species	Avena sativa
Source Germplasm	Brooks CIav9260
Source Tissue	etiolated leaf
PCR size	1.1
Clone Vector	pBluSK
Excision Enzyme	EcoRI XhoI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E.
Clone Authority	Sorrells, Mark E.
Image	CDO1081 BROWS autoradiogram CDO1081 KO autoradiogram CDO1081 SO autoradiogram
Data Source	Sorrells, Mark E. 96.07