GrainGenes Probe Report: CDO1158

[ Printable Version ]  [ Submit comment/correction ]

Probe	CDO1158  [ Marker Report ]
Locus	Xcdo1158-6A CDO1158 Xcdo1158
Synonym	Synonym HAR35_37176   HAR35_6098
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Uni-ZAP XR library OatUni-ZAPXR [c] OatUni-ZAPXR
Type	cDNA
Sequence	BE439208 CDO1158_WHE03F03.r1
Polymorphism	CDO1158 EcoRI   CDO1158 EcoRV   CDO1158 DraI
Linkage Group	6S
Insert Enzyme	EcoRI XhoI
Source Species	Avena sativa
Source Germplasm	Brooks CIav9260
Source Tissue	etiolated leaf
Insert Size	1.5
PCR size	1.5
Clone Vector	pBluSK BS-SK-
Excision Enzyme	EcoRI XhoI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	CDO1158 BROWS autoradiogram CDO1158 KO autoradiogram
Data Source	Sorrells, Mark E. 96.07 ATMI 92.09